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发明公开
CN105785015A 一种快速高灵敏检测大田软海绵酸毒素的试剂盒及方法 无效 - 驳回

一种快速高灵敏检测大田软海绵酸毒素的试剂盒及方法
摘要：
本发明公开了一种快速高灵敏检测大田软海绵酸毒素的试剂盒及方法，属于食品检测领域，特别涉及利用BSA与OA合成偶联物OA?BSA，以及HRP标记OA的单克隆抗体形成HRP?MAb。本发明的试剂盒包括OA?BSA标记的可拆卸8*12的酶标板、OA系列浓度的标准品、HRP标记试剂盒标记的OA单克隆抗体HRP?MAb、10×浓缩洗涤液、显色底物TMB、终止液HCl。本发明方法的检测限为0.19ng/ml，检测范围为0.2?5ng/ml，换算成贝肉中的检测限为9.5ug/kg，检测范围为10?250ug/kg，而进出口标准规定贝类总OA含量不超过160ug/kg，该试剂盒很好的达到了检测要求，同时检测限远低于基于PP2A的试剂盒(50ug/kg)和小鼠生物法(200ug/kg)。另外，本发明试剂盒价格低廉，操作简单，反应时间短，能同时检测84个样品，适用于现场快速检测。
摘要（英）：

The invention discloses a kit and method for rapidly and highly sensitively detecting OA, belongs to the field of food detection, and particularly relates to synthesis of a conjugate OA-BSA with BSA and OA and labeling of a monoclonal antibody of OA with HRP for formation of HRP-MAb. The kit comprises a detachable 8*12 ELISA plate labeled by OA-BSA, a standard at an OA series concentration, an OA monoclonal antibody HRP-MAb labeled by an HRP labeling kit, 10* washing concentrate, a chromogenic substrate TMB and a stopping solution HCl. A detection limit of the method is 0.19 ng/ml, and is converted into a detection limit 9.5 [mu]g/kg of the OA in shellfish meat, a detection range is 0.2 to 5 ng/ml, and is converted into a detection range 10 to 250 [mu]g/kg of the OA in the shellfish meat, the total OA content of shellfish is specified by the import and export standards not to exceed 160 [mu]g/kg, so that the kit well meets a detection requirement, and meanwhile, the detection limit is far lower than those of a PP2A-based kit (50 [mu]g/kg) and mouse bioassay (200 [mu]g/kg). In addition, the kit is low in cost, easy to operate, short in reaction time and applicable to field rapid detection, and 84 samples can be detected at the same time.

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