The invention relates to a seedling culture method, in particular to a one-film double-greenhouse seed seedling culture method for wild alpine roses.A thin film is firstly laid in a built nursery pond, a seedling culture substrate is laid on the thin film, the nursery pond is built in a greenhouse, and a small shed is constructed on the nursery pond.According to the method, soilborne diseases can be effectively prevented, germination of weeds on the ground is effectively prevented, meanwhile, water seepage can be prevented, and an ideal moisture preservation effect is achieved.A double-greenhouse structure can effectively carry out heat preservation and moisture preservation, and it is avoided that condensation water drops on greenhouse roofs scour and damage seeds.The seedling culture method is easy to operate, the requirement for the conditions of facilities is not high, cost is low, the effect is good, the seeds do not need to be subjected to germination accelerating, and the emergence rate can reach 95% or above.