The invention discloses inactivated vaccine for chicken bursa synovialis mycoplasma. The inactivated vaccine comprises antigen and vaccine adjuvant, wherein the antigen is an inactivated YBF-MS1 strain and preserved in China Center for Type Culture Collection, Wuhan University, Wuhan City, the preservation date is 3, March, 2016, and the preservation number is CCTCC M 2016080. By means of the vaccine, a high-level antibody can be generated, the persistent period is long, cross protection performance is achieved, after immunization is completed, the morbidity of chicken bursa synovialis mycoplasma is obviously lowered, and epidemicity of chicken bursa synovialis mycoplasma can be effectively prevented. Domestically, no chicken bursa synovialis mycoplasma vaccine is on the market, it is the key to prevent and control the disease to prepare the inactivated vaccine for chicken bursa synovialis mycoplasma from the chicken bursa synovialis mycoplasma YBF-MS1 strain, and meanwhile market vacancy can be made up.