The invention provides a distribution mode and development regulation method for oil left after binary flooding of a multilayer reservoir. The method comprises the following steps: 1, making fine comprehensive reservoir geophysical researches; 2, making researches on distribution characteristics of the oil left on the plane, between layers and in the layers after binary flooding by utilizing a digital-analogue integrated modeling technology, making researches on influence of dynamic and static factors on the distribution of the oil left after binary flooding, and establishing a distribution mode of the oil left after binary flooding of the multilayer reservoir according to a numerical simulation research result; 3, performing layer series subdivision on the multilayer reservoir by utilizing a layer series subdivision and recombination four-stage optimization method, and designing an optimal streamline regulation scheme for potential digging over the oil left after binary flooding in combination with well pattern and well spacing conditions according to the distribution characteristics and mode of the oil left after binary flooding of the multilayer reservoir. According to the distribution mode and development regulation method for the oil left after binary flooding of the multilayer reservoir, the unit recovery rate after binary flooding of the multilayer reservoir is increased, and the method also has guiding significance for similar domestic and foreign reservoir recovery rate increasing technologies.