The invention relates to application of the combination of hyperoside and oseltamivir to a drug for prevention and treatment of H7N9 influenza and belongs to the field of traditional Chinese medicine application. The invention is implemented by the following technical scheme: firstly, H7N9 wild type or mutant influenza virus neuraminidase liquid and hyperoside and oseltamivir mixed liquid are incubated for 30 minutes, a substrate is added for detection, and an experimental result indicates that the combined use of the two drugs has an inhibiting effect on H7N9 wild type and mutant influenza virus neuraminidase activity; then, the anti-influenza effect of the combined use of the hyperoside and the oseltamivir on cellular levels is detected to discover that the combined use of the hyperoside and the oseltamivir can play a role in treating cells infected by an H7N9 wild type or mutant influenza virus; finally, the effect of the combined use of the hyperoside and the oseltamivir on a mouse influenza model is detected to discover that after a hyperoside and oseltamivir mixture is added, the weight of a influenza mouse can be obviously reduced, so that the mouse can gradually regain health, and the lung indexes of the influenza mouse can be obviously reduced.