The invention provides a cell culture dish and a cell sampling method thereof. The cell culture dish comprises a culture dish hole and sleeves, wherein the culture dish hole is used for culturing to-be-separated cells, the sleeves are placed into the culture dish hole during cell extraction to delimitate the cell extraction area of a target position, the sleeves include a first sleeve, a second sleeve and a third sleeve, the first sleeve is provided with a first sampling hole concentric with the first sleeve, the second sleeve is provided with a second sampling hole which deviates from the circle center of the second sleeve, and the third sleeve is provided with a third sampling hole located at the edge of the wall of the third sleeve. The cell culture dish has the advantages that the cell culture dish is provided with the three sleeves with different sampling hole positions, the target cells at different positions in the cell culture dish can be covered, and sampling purposiveness is increased.