The invention belongs to the field of bio-medical metal materials and particularly relates to a manufacturing method of an antibacterial titanium alloy for bio-medical treatment. A Ti-Al-Fe-Zr-Ag-RE system alpha+beta type two-phase titanium alloy with Ti-5Al-2.5Fe as a basis is prepared through an intermediate alloy, the smelting and production technology of the Ti-Al-Fe-Zr-Ag-RE antibacterial titanium alloy is performed, the Ti-Al-Fe-Zr-Ag-RE antibacterial titanium alloy component range (by weight percent) is provided, titanium alloy theoretical research shows that Al, O and N in the titanium alloy are alpha-phase stable elements, Fe is a beta-phase stable element, Zr is a neutral strengthening element, and the cold and hot processing performance and the plasticity of the titanium alloy are improved; Ag is a co-elution type element, and research at home and abroad shows that silver ions have very strong sterilization performance; a trace of rare earth elements can refine crystalline grains and improve the mechanical performance of the titanium alloy; and multi-element micro-alloy element composite strengthening is the development direction of the titanium alloy.