The invention provides a detection method for listeria monocytogenes (LM). The method comprises the steps of firstly combining the LM with a coated monoclonal antibody, then connecting with a biotinylated polyclonal antibody, next connecting with a streptavidin labeled catalase C100, catalyzing decomposition of hydrogen peroxide by the catalase, reducing fluorescence quenching of mercaptopropionic acid modified cadmium telluride quantum dots, and determining the concentration of the LM in a sample according to the level of the fluorescence intensity. The method is based on the double-antibody sandwich enzyme-linked immunosorbent assay, and a biotin-avidin system is used for amplification of the reaction. More importantly, due to use of the new antibody labeled enzyme (catalase C100) and the more sensitive fluorescent substrate (cadmium telluride quantum dots), and matching of the effective reaction conditions, the detection sensitivity is significantly improved, at the same time, the cost is reduced, and the detection efficiency is improved; therefore, the detection method has good prospects for promotion.