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CN105854084A 一种抗细胞早衰的细胞外基质生物材料及其制备方法和应用 无效 - 撤回

一种抗细胞早衰的细胞外基质生物材料及其制备方法和应用
摘要：
本发明公开了一种抗细胞早衰的细胞外基质生物材料及其制备方法和应用，是培养脐带间充质干细胞分泌的基质，该基质可抗细胞的早衰。将脐带间充质干细胞培养在完全培养基中，当细胞密度接近90%时，加入含有抗坏血酸的完全培养液培养7?10天；然后加入脱细胞液，构建得到细胞外基质生物材料。所述脐带间充质干细胞取自人源、猪源、鼠源或兔源。本发明提供的抗细胞早衰的细胞外基质生物材料可用于细胞的培养，包括细胞移植治疗，再生医学和组织工程等领域。本发明研究表明MSCs培养在ECM的环境下胞内ROS的含量明显下降，证实了来源于UC?MSCs的ECM能够缓解H2O2诱导的UC?MSCs早衰，为解决细胞衰老带来的挑战提供了一种新的策略。
摘要（英）：

The present invention discloses an extracellular matrix biomaterial for resisting cell premature senility and a preparation method and application thereof. The matrix is secreted from culture of umbilical cord mesenchymal stem cells and is resistant to cell premature senility. The umbilical cord mesenchymal stem cells are cultured in a complete medium; when the cell density is close to 90%, a complete culture solution containing ascorbic acid is added, and culture is carried out for 7-10 days; then a cell removal solution is added to construct an extracellular matrix biomaterial. The umbilical cord mesenchymal stem cells are obtained from human source, swine source, murine source or rabbit source. The extracellular matrix biomaterial for resisting cell premature senility can be applied to cell culture, including cell transplantation therapy, regenerative medicine and tissue engineering, etc. Studies show that the intracellular MSCs cultured in ECM environment has ROS content significantly decreased, which confirms that ECM from UC-MSCs can alleviate the UC-MSCs premature senility induced by H2O2 and provide a new policy to address the challenges brought by cells aging.

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