Provided is a glass-lined detachable stirrer. The glass-lined detachable stirrer comprises a stirring vane assembly, a seal holder, seal rings, a stirring shaft, locating pins, a connecting screw, a gasket and a lock nut; after the lock nut is tightened, the stirring vane assembly is closely connected with the stirring shaft, the seal rings on the seal holder have a good sealing effect, and a corrosive medium is prevented from intruding. Meanwhile, due to the fact that the seal holder is soft in texture, when the connecting screw is subjected to pressing force, the seal holder can produce slight deformation, a first end pressing face on the stirring vane assembly makes contact with and is pressed with a second end pressing face on the stirring shaft, continuous deformation of the seal holder is prevented, and in the rotating process of the stirrer, the stirrer is not subjected to additional compressive force any more. By means of the locating pins, relative motion between the stirring vane assembly and the stirring shaft can be effectively prevented, and an anti-reverse device does not need to be particularly arranged on a transmission device.