The invention provides keratinase-producing Pseudomonas beteli of which the collection number is CGMCC No.12183. The invention also provides application of the keratinase-producing Pseudomonas beteli in degrading keratin. After fermentation culture is carried out for 24 hours, the degradation rate for feather can reach 60%, and the keratinase activity reaches 35.6 U/mL. The keratinase-producing Pseudomonas beteli has considerable application prospects in the field of degradation and reutilization of keratinase-containing livestock/fowl waste.