The invention discloses a biological cell sap test box provided with a filter membrane. The biological cell sap test box comprises a box body, the box body is provided with a cover and a body bottom shell, the body bottom shell is internally provided with a fixing layer, and the top face of the fixing layer is an inclined face; the fixing layer is internally provided with a plurality of liquid channels from top to bottom; a glass slide layer is arranged below the fixing layer, a CCD sensor is arranged below the glass slide layer, and a circuit layer is arranged below the CCD sensor; the circuit layer is internally provided with a test circuit, the inclined face of the fixing layer is arranged, detection liquid drops are placed at the highest point, the detection liquid can flow downwards along the inclined face and enters different liquid channels, the glass slide layer below the detection liquid intercepts the detection liquid, the detection liquid is observed, observation and recording of multiple sets of detection liquid can be achieved at the same time, the biological cell sap test box is convenient to carry, and it is convenient to detect in real time.