The invention relates to a cell extraction method in the technical field of bioengineering, in particular to an extraction method for chick embryo myoblasts. The method includes the following steps that firstly, chick embryos are sterilized, and pectoralis major is taken and put into a D-Hank's culture dish; secondly, the pectoralis major is trimmed; thirdly, the pectoralis major is oscillated and suspended through a vortex oscillator; fourthly, filtration and centrifugation are carried out; fifthly, cell plate laying and differential attachment are carried out; sixthly, digestion is carried out to obtain the myoblasts; seventhly, counting and plate planting are carried out. By means of the extraction method for the chick embryo myoblasts, namely, the mechanical oscillation extraction method for the chick embryo myoblasts, it is guaranteed that the purity, number and vitality of the extracted myoblasts are higher than those of myoblasts extracted through other methods, and a foundation is laid for further development of poultry muscle development regulation and control mechanism experiments.