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发明公开
CN105891383A 同时检测烟草及烟草制品中烟草特有亚硝胺和挥发性N-亚硝胺的GC-MS/MS方法 无效 - 撤回

同时检测烟草及烟草制品中烟草特有亚硝胺和挥发性N-亚硝胺的GC-MS/MS方法
摘要：
一种同时检测烟草及烟草制品中烟草特有亚硝胺和挥发性N?亚硝胺的GC?MS/MS方法，具体包括四种烟草特有亚硝胺：NNN、NAT、NAB、NNK，和八种挥发性N?亚硝胺：NDMA、NPYR、NPIP、NMOR、NEMA、NDEA、NDPA、NDBA；其特征在于：在烟草样品中加入氢氧化钠溶液，手持振荡混合均匀后加入二氯甲烷，振荡器振荡，加入无水硫酸镁，手持振荡后置于振荡器中振荡，然后离心、浓缩，最后用GC?MS/MS分析。本发明的优点在于：样品前处理简便、烟草特有亚硝胺和挥发性N?亚硝胺能够同时被分析，可批量处理，且该方法测定速度快、加标回收率好、精密度高，可为准确评估烟草及烟草制品中烟草特有亚硝胺和挥发性N?亚硝胺的安全风险提供技术支撑。
摘要（英）：

The invention discloses a GC-MS/MS method for synchronously detecting specific nitrosamine and volatile N-nitrosamine in tobacco and tobacco products. Four kinds of nitrosamine of NNN, NAT, NAB and NNK and eight kinds of volatile N-nitrosamine of NDMA, NPYR, NPIP, NMOR, NRMA, NDEA, NDPA and NDBA are specially included. The method is characterized in that a sodium hydroxide solution is added into a tobacco sample, dichloromethane is added after uniform mixing carried out in a handholding oscillation mode, oscillation is carried out through an oscillator, anhydrous magnesium sulfate is added, the mixture is added into the oscillator to be oscillated after handholding oscillation, then, centrifugation and concentration are carried out, and finally, GC-MS/MS analysis is carried out. The method has the advantages that sample pretreatment is easy and convenient, the specific nitrosamine and volatile N-nitrosamine in the tobacco can be analyzed synchronously, batch treatment can be carried out, the measuring speed is high, the adding standard recovery rate is good, the degree of precision is high, and the technical support can be provided for accurately assessing the safety risks of the specific nitrosamine and volatile N-nitrosamine in the tobacco and tobacco products.

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