The invention relates to an enrichment method for detecting hydrophilic amino acid and belongs to the technical field of analytical chemistry detection. According to the enrichment method, after a sample containing hydrophilic amino acid is extracted and dissolved by virtue of an organic solvent in a certain proportion, silica gel surface polar group bonded phase filler is utilized for preparation; by optimizing parameters such as solvent dosage and leaching proportion in the preparation process, the enrichment preparation of hydrophilic amino acid in the sample is realized; and the prepared amino acid sample can be simultaneously detected once under certain chromatography and mass spectrometry conditions. The method has the characteristics that the stability is good, the recovery rate is high, the operation is simple, convenient and controllable, and the like.