The invention provides a pharmaceutical preparation for prevention and treatment of chronic respiratory diseases of livestock and poultry. The pharmaceutical preparation is mainly prepared from, by mass, 15-30 parts of antibiotics, 4-10 parts of herbal epimedii, 4-10 parts of radix bupleuri, 8-18 parts of folium eriobotryae, 3-6 parts of fructo-oligosaccharide, 20-30 parts of medical stone and 15-25 parts of anhydrous dextrose. A preparation method comprises the steps that herbal epimedii, radix bupleuri, folium eriobotryae, fructo-oligosaccharide and medical stone are mixed and smashed, the mixture passes through a screen of 150 mesh or above, antibiotics are added, the mixture continues to be mixed and stirred, anhydrous dextrose is added, and the mixture is stirred to be uniform. According to the embodiment, the pharmaceutical preparation for prevention and treatment of chronic respiratory diseases of livestock and poultry is mild in property and small in side effect, compatibility among the raw materials is utilized for repairing immune cells of livestock and poultry, and meanwhile growth of normal flora in intestines is protected and promoted, so that the immunologic function of an organism is enhanced.