The invention provides a micro dispensing device suitable for narrow slits. The micro dispensing device and method suitable for narrow slits comprises a drive mechanism, a liquid transfer needle regulating mechanism and a micro pipe regulating mechanism. The drive mechanism comprises a supporting frame, a rotary motor, a coupling, an eccentric gear mechanism and a liquid transfer needle mounting plate, wherein the rotary motor, the coupling, the eccentric gear mechanism and the liquid transfer needle mounting plate are installed on the supporting frame; and the liquid transfer needle mounting plate is connected with the eccentric gear mechanism. The output end of the rotary motor is connected with the eccentric gear mechanism through the coupling in a driving mode. The liquid transfer needle regulating mechanism comprises a liquid transfer needle clamp and a liquid transfer needle, and the liquid transfer needle clamp and the liquid transfer needle are mounted on the liquid transfer needle mounting plate. The micro pipe regulating mechanism comprises a micro pipe support and a micro pipe fixed to the micro pipe support. The micro pipe support comprises a micro regulating module and a posture regulating component. The invention further discloses a micro dispensing method suitable for narrow slits. The micro dispensing device and method are suitable for micro dispensing of narrow slits of various viscosities, the dispensing process is simple and easy to implement, and the micro dispensing device and method further have the advantages of adjustable frequency (with maximum frequency being 130 Hz), the super micro volume (the fL level), narrow slit dispensing and the like.