The invention discloses an automatic calibration and detection punching machine used for performing punching treatment on an FPC. The automatic calibration and detection punching machine comprises an uncoiling device used for performing uncoiling feeding on the FPC, a conveying device used for conveying the FPC, a clamping device used for clamping and fixing the FPC, a position adjusting device used for adjusting the position of the conveying device, a punching device used for performing punching treatment on the FPC, and a recoiling device used for performing recoiling material receiving on the FPC, and the conveying device is in driving connection with the clamping device. The automatic calibration and detection punching machine achieves automatic punching operation on the FPC, and therefore the labor cost is reduced, and the efficiency is greatly improved. Before punching, the punching position of the FPC can be automatically calibrated, and therefore the punching precision is guaranteed, punching detection can be performed on the FPC after punching is completed, and therefore the percent of pass of production of products is increased.