The invention discloses agaricus bisporus endophyte and an application thereof. The agaricus bisporus endophyte is lysinibacillus, and is named as lysinibacillus SCAU1, and the deposited number is CCTCCM2016106. The agaricus bisporus endophyte is functional endophyte separated from agaricus bisporus, promotes growth of plants, inhibits growth of plant pathogenic fungi and pathogenic bacteria, has efficient cellulose degradation capability, and can be used for preparing a 'fertilizer-pesticide double-effect' biofertilizer, the physical and chemical properties of soil are improved, and growth of the plants is promoted; three continuous pot experiments show that after SCAU1 fermentation liquor is diluted with clear water, a growth promoting effect on corn seedlings is obvious by a root irrigation mode, and the number, length, height, blade number and leaf area of corns in the seedling stage can be increased obviously; and flue-cured tobacco is taken as a supplied experimental plant, a field experiment for SCAU1 is carried out, a result shows that stem thickness, height and leaf area coefficients of the flue-cured tobacco in the 87 resettling stage can be increased, and the yield and the potassium content of the flue-cured tobacco are increased.