The invention belongs to the technical field of industrial microorganisms, and relates to a method for intensively culturing biological demulsifying bacteria by adopting a biological surfactant tea saponin. The method mainly comprises the following steps that the tea saponin within the certain concentration range is added in the initial stage of fermentation; the biomass and the demulsifying efficiency of the biological demulsifying bacteria are evaluated after fermentation is finished; the tea saponin concentration at which production promoting and efficiency improving of the biological demulsifying bacteria can be simultaneously and significantly achieved is preferably selected. According to the method, the adopted tea saponin has the advantages of being high in surface activity and ecological friendliness, low in toxicity, low in cost, capable of being easily obtained and the like, can play a significant promoting role in the biomass and the demulsifying efficiency of the biological demulsifying bacteria at the low dosage and can reduce the synthesizing cost of a demulsifying agent and provide a simple and effective intensifying strategy for demulsifying agent production to lay a foundation for solving the bottleneck problems that in demulsifying agent application, the yield is low, and the cost is high.