利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN105907884A 水稻抗稻瘟病基因Pita特异性分子标记引物及应用 有权 许可

水稻抗稻瘟病基因Pita特异性分子标记引物及应用
摘要：
本发明公开了水稻抗稻瘟病基因Pita特异性分子标记引物及应用。申请人通过将携带不同Pita等位基因的水稻品种测序及序列比对在Pita编码区内含子鉴定到一个Pita特异性位点并开发了一套鉴定该目标位点的分子标记引物，分别为：SF：GCAGGTTATAAGCTAGCTAT；SR：GCCAAATAGCCAATTCATA；RF：CAAGAGAGAGTATTTGCTTAGGAGT；RR：CCTATATCTCTTTAAAATATGTTTGGC。利用该引物对水稻种质资源DNA进行PCR扩增，可快速判断待测试水稻Pita的基因型，从而将携带抗性等位基因的材料应用于生产。方法简便快速、成本低廉，可广泛应用于分子标记辅助选择育种或者水稻种质资源Pita基因型鉴定。
摘要（英）：

The invention discloses a rice blast resistant gene Pita specific molecular marker of rice and an application. By sequencing a rice variety with different Pita allelic genes and aligning sequences, the applicant identifies one Pita specific lotus in a Pita coding region intron, and develops a set of molecular marker primers for identifying the target lotus, wherein the molecular marker primers are separately as follows: SF: GCAGGGTTATAAGCTAT; SR:GCCAAATAGCCAATTCATA; RF:CAAGAGAGAGTATTTGCTTAGGAGT; RR: CCTATATCTCTTTAAAATATGTTTGGC. The primers are utilized to carry out PCR amplification on DNA of rice genetic resources, so that the gene type of to-be-tested rice Pita can be quickly judged, and therefore, a material with resistant allelic genes is applied to production. The method is simple and quick, is low in cost, and can be widely applied to molecular marker-assisted selective breeding or rice genetic resource Pita gene type identification.

公开/授权文献
0/0