The invention belongs to the field of preparing of a TiO2 nanotube array, and particularly relates to a preparing method of the TiO2 nanotube array in a controllable three-dimensional shape. According to the method, roughness of a titanium substrate surface is changed firstly, and an electrochemistry anodic oxidation method is used for constructing the TiO2 nanotube array in the controllable shape on the titanium substrate surface; and the method specifically comprises following steps: a metal titanium substrate is thoroughly cleaned firstly, a chemical corrosive liquid capable of changing roughness is prepared, and substrate materials of different kinds of roughness can be obtained by controlling corrosion time of the titanium substrate in the corrosive liquid; according to a prepared electrolyte, the solute is ammonium fluoride, a solvent is ethylene glycol, and according to the mass percent, the content of the ammonium fluoride accounts for 0.25% of the total amount of the electrolyte; and graphite is used for carrying out electrochemistry anodic oxidation on an electrode, a TiO2 nanotube array film of different three-dimensional structures can be obtained on the titanium substrates with the different kinds of roughness, the structure is in order, the shape is controllable, the pipe length and the pipe thickness are controllable, and the formed nanotube film is stable in structure and is not prone to falling off.