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发明公开
CN105911284A 一种基于微球的杯式时间分辨荧光肌酸激酶同工酶分析试剂盒及其制备方法和应用 无效 - 驳回

一种基于微球的杯式时间分辨荧光肌酸激酶同工酶分析试剂盒及其制备方法和应用
摘要：
本发明属于临床医学诊断领域，特别涉及一种基于微球的杯式时间分辨荧光肌酸激酶同工酶分析试剂盒及其制备方法和应用,该试剂盒由检测反应杯、荧光标记抗体及清洗液组成。在具体检测时，先绘制标准曲线，然后将待测样品加入检测反应杯内，再加入荧光标记抗体，30℃?40℃温育，用清洗液清洗去除未结合的抗原及荧光标记抗体；然后将荧光信号与标准曲线比对，获得所述待测样品的肌酸激酶同工酶浓度。所述肌酸激酶同工酶的检测方法，由于其超高的灵敏度，易于实现自动化操作，而且反应温度均一，不受环境因素影响，准确性更好，精密度也非常优异。
摘要（英）：

The invention especially relates to a cup-type time-resolved fluorescent analysis kit for creatine jubase-MB based on microspheres, and a preparation method and application thereof, belonging to the field of clinical medical diagnosis. The kit is composed of a detection reaction cup, a fluorescence labeled antibody and a cleaning solution. In concrete detection, a standard curve is drafted at first, then a to-be-detected sample is added into the detection reaction cup, then the fluorescence labeled antibody is added, incubation at 30 to 40 DEG C is carried out, and unbonded antigen and fluorescence labeled antibody are removed through cleaning via the cleaning solution; and the fluorescence signals are compared with the standard curve so as to obtain the concentration of creatine jubase-MB in the to-be-detected sample. The detection method for the creatine jubase-MB is easy to realize automatic operation due to its superhigh sensitivity, uniform in reaction temperature, free of influence by environmental factors, better in accuracy and excellent in precision.

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