The invention discloses a method for fast inducing bottle cultured pleurotus nebrodensis fruitbody formation and improving the single mushroom bud rate. The method comprises the following steps that the first hole point position in the middle of a material surface is subjected to material surface mycelium stimulation; a bottle cap is opened; old strains are clearly cleaned; an iron rod with the diameter being 0.5cm to 1cm is used for performing mycelium stimulation for one point hole in the material surface, and the depth is 1cm to 1.5cm; or the perforation mycelium stimulation is performed in the second hole point position on the material surface; the bottle cap is opened; old strains are not cleaned; the iron rod with the diameter being 0.5cm to 1cm is used for forming a second point hole on the material surface, and the depth is 1cm to 1.5cm. After the mycelium stimulation, the formation of mushroom buds is early; few material surface mushroom buds are formed; the single mushroom bud rate can reach more than 75 percent to the highest degree; the manual bud thinning workload is greatly reduced.