The invention discloses application of 183aa micro-molecule polypeptides to preparation of anti-cancer drugs. By means of the bioengineering technology, a section of DNA sequence corresponding to 183 amino acid polypeptides is genetically recombined to a p3*FLAG-CMV-14 eukaryotic expression vector. Digestion and sequence analysis prove that after recombination is successful, the recombined polypeptides are transfected to multiple myeloma RPMI8226 cells, the protein expression of the polypeptides is detected through western blot, and recombination of the polypeptides is achieved. Then, studying is carried out on the function of the polypeptides to resist multiple myeloma, and cytology experiments show that the polypeptides have an important anti-cancer function of promoting myeloma cell apoptosis. An experiment basis is provided for new target spot developing on oncotherapy, and the 183aa micro-molecule polypeptides have very great development and application prospects when applied to tumorous clinic treatment.