The invention relates to the field of mushroom cultivation, and specifically provides a cultivation method for mushrooms capable of calming nerves by utilizing mixed manures. The cultivation method replaces cattle manure in a traditional mushroom culture medium with mixed manure residues to cultivate the mushrooms. The method comprises the following production process flows: different manures, solid-liquid separation, different manure residues, mixing of the different manure residues, culture medium preparation, stacking material pre-wetting, pre-fermentation, after fermentation, cooling, sowing, mycelium germination and growth and soil covering, and fruiting, wherein the mixed manure residues comprise the following components by mass, in terms of 1500 kg of the mixed manure residues, 500 kg of cattle manure residue, 400 kg of pig manure residue, 300 kg of sheep manure residue, 200 kg of chicken manure residue and 100 kg of duck manure residue; and pine-needle residues and pine juice are prepared through the following steps: 1) raw material preparation; 2) crushing; 3) stewing; and 4) filtering. The cultivation method provided by the invention realizes resource utilization of the pig manure, the cattle manure, the sheep manure, the chicken manure, the duck manure, etc.; meanwhile, the mushroom cultivated by using the cultivation method provided by the invention has a certain function of calming the nerves.