The invention discloses a preparing and collecting method for starch particles. The preparing and collecting method comprises the steps that starch is subjected to superfine grinding and then prepared into a starch solution with a certain concentration; the starch solution is subjected to homogenization with a high-pressure homogenizing machine and treated with a high-speed fluidizing device and then passes through a super centrifuge, centrifugal separation is carried out at different rotating speeds, and starch particles of different sizes are obtained; finally, the starch solution containing starch particles of different sizes is led into an electrophoresis tank, a cationic surfactant is added, polar plates in different shapes or with specific patterns are arranged, and starch particles assembled in a patterned mode in different sizes are obtained on a collector. According to the preparing method, the starch particles obtained after superfine grinding in combination with high-speed fluidizing are obvious in damage of crystalline structures, active groups are fully exposed, the action between the starch particles and the cationic surfactant is easier, and the collected starch particles with different patterns and different sizes are formed at a time and uniform and can be widely applied in the fields of preparing functional starch films, patterned paper and the like.