The invention relates to the technical field of agriculture, in particular to a breeding method of a maize inbred line suitable for being used as a transformation receptor. The breeding method comprises the following steps: performing hybridization by using a maize variety which easily establishes a transformation system as a female parent and using a maize variety with excellent agronomic characters as a male parent, taking immature embryos of hybridized clusters for heredity transformation, selecting the hybridized clusters with excellent agronomic characters of plants from the hybridized clusters with high heredity transformation efficiency, sowing seeds of the hybridized clusters, and performing inbreeding; selecting cluster immature embryos of plants with excellent agronomic characters for heredity transformation, sowing inbred cluster seeds with high heredity transformation efficiency, and performing inbreeding; repeating the previous step. The inbred line bred by the breeding method disclosed by the invention integrates the relevant advantages of parents, and is easy to regenerate, good in stress resistance, high in heredity stability and good in agronomic characters; the technical defects of low heredity transformation efficiency and the like of conventional acceptor materials are effectively overcome, the heredity transformation system is easy to establish, and a new receptor is provided for genetic modification of maize.