The invention discloses an efficient breeding method of a frog male tetraploid pure line. The method comprises the following steps: preparing tamoxifen feed; feeding a female frog with the tamoxifen feed; treating through cold shock and hydrostatic pressure; preparing an estrogen inhibitor solution; putting a zygote into the estrogen inhibitor solution; and after tadpoles are obtained through normal incubation, fetching the tadpoles and feeding with clomiphene larva feed and the like. Through the method, 100% frog male tetraploid pure line can be obtained, and the breeding rate and emergence rate of tetraploid are effectively increased; and compared with hybridization, a male tetraploid fish system with higher purity and more stable offspring can be obtained, and a firm foundation is laid for the breeding of infertile triploid frogs.