The invention discloses a ricefield eel catching, killing and dissecting device which comprises an elastic plate and a dissecting knife, the two ends of the elastic plate are arranged to be a first buckling end and a second buckling end respectively, a grasping cavity which is provided with a gap in the side portion and openings in the two ends is enclosed between the first buckling end and the second buckling end, the gas allows ricefield eels to be placed into the grasping cavity, a gap is formed between the end face of the first buckling end and the end face of the second buckling end, and the end face of the first buckling end and the end face of the second buckling end form a sliding face which is used for grasping the ricefield eels and allowing the ricefield eels to slide in the direction of the connection line of the openings in the two ends of the grasping cavity; the elastic plate provides elastic stress for the first buckling end and the second buckling end to be away from each other; the dissecting knife is fixed to the elastic plate, and the tip of the dissecting knife faces inwards the grasping cavity and is used for being in abutting fit with the ricefield eels. The ricefield eel catching, killing and dissecting device is convenient to operate, high in safety and capable of greatly improving work efficiency.