The invention provides a walking mechanism trolley assembly line. The walking mechanism trolley assembly line comprises a cleaning area used for cleaning a wheel set in advance, an automatic assembling area used for automatically assembling the wheel set, and a manual auxiliary assembling area. The cleaning area comprises a dust removing device for cleaning special machines. The automatic assembling area is connected with the cleaning area in series through a roller bed. The manual auxiliary assembling area comprises a trolley frame, a speed reducer, a motor support, a guard bar and a wheel clamping device. The special machines and the wheel set are adopted in the trolley frame. The rib line layout is adopted in the speed reducer, the motor support, the guard bar and the wheel clamping device. The walking mechanism trolley assembly line overcomes existing assembling technical problems, and an assembly line high in automation degree and low in labor intensity of workers is provided. Various special machines, mechanical arms, shuttle vehicles and other advanced devices are utilized in the walking mechanism trolley assembly line for replacing traditional manual work.