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Invention Publication
CN105950735A 一种用于区分鉴别感染不同种牛泰勒虫的试剂盒 无效 - 驳回

一种用于区分鉴别感染不同种牛泰勒虫的试剂盒
Abstract:
本发明公开一种用于能够检测与区分牛环形泰勒虫、瑟氏泰勒虫和中华泰勒虫的定量PCR检测引物和探针。本发明的引物对及探针的基因序列分别为：SEQ ID NO.1/SEQ ID NO.2和SEQ ID NO.3、SEQ ID NO.4/SEQ ID NO.5和SEQ ID NO.6、SEQ ID NO.7/SEQ ID NO.8和SEQ ID NO.9，并分别在探针的5’端标记了荧光基团FAM、在3’端标记了荧光淬灭基团BHQ1。本申请中建立的检测牛环形泰勒虫、瑟氏泰勒虫和中华泰勒虫的定量PCR方法能够很好补充显微镜检测的缺点，方便的检测区分这三种泰勒虫，具有灵敏度高、特异性强的优点，并能够定量的检测感染寄生虫DNA。
Abstract(English):

The invention discloses a quantitative PCR (polymerase chain reaction) detection primer and a probe for detecting and distinguishing theileria annulata, theileria sergenti and theileria sinensis. The gene sequences of the primer and the probe are respectively SEQ ID NO:1/SEQ ID NO:2 and SEQ ID NO:3, SEQ ID NO:4/SEQ ID NO:5 and SEQ ID NO:6, and SEQ ID NO:7/SEQ ID NO:8 and SEQ ID NO:9, wherein the 5'terminals of the probe are marked with fluorescent genes FAM, and the 3' terminals are marked with fluorescent quenching genes BHQ1. The quantitative PCR method for detecting the theileria annulata, theileria sergenti and theileria sinensis has the advantages that the disadvantages of microscope detection are well supplemented, the theileria annulata, theileria sergenti and theileria sinensis can be conveniently detected and distinguished, the sensitivity is high, the specificity is strong, and the DNA (deoxyribose nucleic acid) of infectious parasite can be quantitatively detected.

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