The invention relates to the technical field of detection of a traditional Chinese medicinal material, and particularly discloses an HPLC fingerprint spectrum of a pouzolzia zeylanica var. microphylla medicinal material or general flavonoids of pouzolzia zeylanica var. microphylla and a building method and application of the HPLC fingerprint spectrum. The building method comprises the following steps: S1, precisely weighing a pouzolzia zeylanica var. microphylla medicinal material to prepare a to-be-tested solution; and S2, enabling the to-be-tested solution to be subjected to high performance liquid chromatography to obtain a fingerprint spectrum. The fingerprint spectrum can overall reflect information of characteristic peaks of a reaction sample, and the method is good in stability and reproducibility. The fingerprint spectrum can be used for true and false or quality identification of the pouzolzia zeylanica var. microphylla medicinal material or general flavonoids of pouzolzia zeylanica var. microphylla.