The invention discloses a pharmaceutical composition resistant to drug-resistance bacteria and a preparing method and application thereof. The pharmaceutical composition is a refined product prepared from a pharmaceutical composition obtained through compatibility of honeysuckle flower and fructus forsythia. The pharmaceutical composition is prepared from, by weight, 0-10 parts of honeysuckle flower and 0-10 parts of fructus forsythia. The pharmaceutical composition is subjected to primary refining and secondary refining to generate optimal active components. Experimental study results show that the product obtained through two times of refining has remarkable antibacterial activity and has unique antibacterial advantages compared with traditional antibiotics, a candidate drug is provided for complete killing of drug-resistance bacteria clinically, and the existing technique is enriched.