The invention relates to an oily titanium-steel isolation coating and application thereof, belongs to the technical field of titanium-steel bonding prevention coatings and aims at providing an oily titanium-steel isolation coating used in titanium tube rolling. The coating is prepared from, by weight, 50-150 parts of an oily film-forming agent, 350-400 parts of aluminum powder, 0-50 parts of talcum powder, 0-50 parts of iron powder and 400-450 parts of aluminum oxide powder. The oily titanium-steel isolation coating is applied to a steel and iron device, a roller or a winch to form a very good isolation layer and can ensure that titanium ingots do not produce bonding reaction with the steel and iron device, the roller or the winch in the 900 DEG C rolling or perforation process, improve the size precision and surface quality of products, improve the machining yield and production efficiency of titanium materials and reduce the production costs.