The invention discloses a novel phytase mutant and belongs to the field of biotechnology. The mutant shows excellent characteristics in aspects of temperature tolerance, enzyme activity and the like, thereby having a relatively good application prospect. After the mutant is induced by methanol for 96 hours, the enzyme activity of fermentation liquor is up to 28,000U/mL, which is higher than that of an original phytase by 180%; more than 90% of the enzyme activity is kept after the phytase is processed at the temperature of 75 DEG C; the mutant can tolerate the pelletizing temperature of 85 DEG C, and the remaining enzyme activity is more than 85%, thereby meeting the production requirements of pellet feeds.