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发明授权
CN105969875B 用于检测微量组织中PIK3CA基因突变的引物组合及其应用 有权

用于检测微量组织中PIK3CA基因突变的引物组合及其应用
摘要：
本发明公开了一种用于检测微量组织中PIK3CA基因突变的引物组合，其包括预扩增引物组、用于检测9号外显子突变的引物组、用于检测20号外显子突变的引物组；该方法旨在将微量的DNA通过预扩增得到较高浓度的DNA模板，结合直接测序法检测样本中PIK3CA基因的突变情况；将该引物组合应用于制备检测PIK3CA基因突变的检测试剂中，可检测样本的DNA浓度低至100pg，且可以同时检测PIK3CA基因第9和20号外显子的所有突变，本发明提供的检测方法灵敏度高，特异性强，成本低，适用于临床肿瘤患者PIK3CA基因突变的检测，具有很好的临床应用价值。
摘要（英）：

The invention discloses a primer combination for detecting mutation of a PIK3CA gene in trace tissue. The primer combination comprises a pre-amplification primer group, a primer group for detecting No.9 exon mutation and a primer group for detecting No.20 exon mutation. According to a method, a trace amount of DNA is pre-amplified to obtain a high-concentration DNA template, and the mutation condition of the PIK3CA gene in a sample is detected by combining a direct sequencing method; by applying the primer combination to preparation of a detection reagent for detecting mutation of the PIK3CA gene, the DNA concentration of the detectable sample is low up to 100 pg, and all mutation of a No.9 exon and a No.20 exon of the PIK3CA gene can be simultaneously detected. The detection method is high in sensitivity and specificity, low in cost and suitable for PIK3CA gene mutation detection on a clinical tumor patient and has the very good clinical application value.

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