The invention discloses an integrated method for implementing latent fingerprint development and transfer based on polydopamine film interface separation. The method comprises the following steps: (1) coating one side of a PDMS (Polydimethylsiloxane) film with a PDA (Polydopamine) film to form a composite film; (2) performing aging pretreatment on a carrier carrying latent fingerprints, then lightly attaching the carrier to the composite film in a mode that the PDA film is used as a contact face, standing for 5s-20min, and then separating the PDMS film from the PDA film, wherein the PDA film and latent fingerprint contact part is left on the PDMS film marked as a transfer film, and the other part is left on the carrier; and (3) separately performing electroless deposition of silver on the transfer film and the carrier to obtain a latent fingerprint image with matched male and female sides. By adopting the method, the latent fingerprints can be developed on different surfaces, and can also be transferred to the PDMS surface for sampling and storage, so that the method has huge application potential in scene fingerprint extraction and the like.