The invention discloses a dustproof moistureproof bending-resistant RS232 serial port data line, and the data line comprises a cable, a USB connector disposed at one end of the cable, and an RS232 connector which is disposed at the other end of the cable and is provided with a fixed bolt. The cable is sequentially provided with an external sheath, an external insulating layer, an external enhancement layer, an external shielding layer and a plurality of conductors from the outside to the inside. A filling material is disposed between the external shielding layer and the conductors. Each conductor is sequentially provided with an internal insulating layer, an internal enhancement layer, an internal shielding layer and a metal cable core from the outside to the inside. The data line also comprises a connector protection sleeve which is used for protecting the USB connector and an RS232 connector. One end of the connector protection sleeve is provided with a USB interface which is used for the insertion of the USB connector, and the other end of the connector protection sleeve is provided with an RS232 interface which is used for the insertion of the RS232 connector. The data line effectively prolongs the service life, and effectively improves the stability of data transmission.