The invention provides a reinforced crude oil distillation agent for distillation of Russian crude oil, and a preparation method and application thereof. The reinforced crude oil distillation agent comprises an activation main body, an auxiliary agent and a solvent, wherein the activation main body is a polyethylene glycol nonionic surfactant; the auxiliary agents comprises three auxiliary agent components; a first auxiliary agent component is a quaternary ammonium salt cationic surfactant; a second auxiliary agent component is a polyol ester non-surfactant; a third auxiliary agent component is phenol; the solvent aromatic hydrocarbons enriched oil; the activation main body accounts for 40 to 70% of the weight of the reinforced crude oil distillation agent; the first auxiliary agent component accounts for 5 to 20% of the weight of the reinforced crude oil distillation agent; the second auxiliary agent accounts for 5 to 15% of the weight of the reinforced crude oil distillation agent; the third auxiliary agent accounts for 5 to 10% of the weight of the reinforced crude oil distillation agent; and the solvent accounts for 10 to 30% of the weight of the reinforced crude oil distillation agent. The reinforced crude oil distillation agent is simple in composition, easy to prepare, low in cost, small in addition amount and capable of improving yield of wax oil and quality of an oil product.