The invention relates to a method for creating intergeneric hybridization of radish and turnip and belongs to the technical field of biotechnology breeding. The method specifically includes: sowing parent materials; performing cross pollination during the flowering stage; checking seeds; performing tissue culture to obtain hybrid seedlings; domesticating aseptic seedlings; performing hybrid morphological identification; performing hybrid cytological identification. The method has the advantages that according to the features that the distant hybridization of the radish and Brassica is severe in pre-fertilization barrier and minor in barrier after pollination, bud pollination is used and glycine of certain concentration is smeared to female parent stigmas before pollination to improve the compatibility of the intergeneric hybridization, natural pod bearing is used after the pollination, seed sterile culture is performed by the aid of the tissue culture technology, and the morphological and cytological identification of the obtained hybrid seedlings shows that the hybrid seedlings are actual intergeneric triploid hybrids.