The invention discloses a rapid breeding method for myrciaria dubia tissue culture. The method includes the following steps that an annual myrciaria dubia branch with nodes is taken and used as an explant; the explant is sterilized and induced; adventitious budpropagation is carried out on axillary buds obtained after inducing; the proliferous axillary buds are cultured to obtain strong seedlings; the strong seedlings are cultured to take root so as to obtain tissue culture seedlings, and the tissue culture seedlings can be transplanted. The technical method of myrciaria dubia tissue culture and rapid breeding is achieved for the first time, and a new path is opened up for the myrciaria dubia tissue culture technology. The rapid breeding method is simple, convenient, easy to implement, short in culture time and capable of effectively shortening myrciaria dubia breeding time and increasing economic benefits. A culture medium and reagents used in the method are easy to prepare and low in cost, and the method has good application prospects.