The invention discloses an anti-puncture polyurethane waterproof coating which consists of a component A and a component B, wherein the component A consists of a prepolymer 1 and a prepolymer 2; the prepolymer 1 consists of 25.8-28.5 parts of toluene diisocynate, 20-30 parts of polyether 220, 20-30 parts of polyether 3050 and 15-25 parts of polyether 330N; the prepolymer 2 consists of 24.3-27.5 parts of toluene diisocynate, 55-65 parts of polyether 220 and 10-20 parts of polyether 3050; and the component B consists of 15.7-17 parts of 3,3'-dichloro-4,4'-diaminodiphenyl methane, 70-80 parts of chlorinated paraffin, 66-80 parts of talc powder, 25-30 parts of dioctyl phthalate, 1-2 parts of stannous octoate and 2-6 parts of antifoaming agent AC301. In the invention, with a multi-crosslinking structure, the rising intensity of the cohesive energy of polyurethane is increased, and the anti-puncture ability is improved.