The invention relates to application of fungus hydrophobin HGFI to trypsin immobilization. Firstly, specific protein in the fungus hydrophobin is used for achieving immobilization of trypsin, wherein the efficient self-assembly film formation and immobilization capabilities of the hydrophobin HGFI are fully utilized, the surface of a polystryrene material is modified through the fungus hydrophobin HGFI via a static adsorption method, the trypsin is immobilized to the surface, modified by the HGFI, of the polystryrene through the static acting force, the hydrophobicity and the biocompatibility of the polystyrene are improved through the biological source performance and the high surface activity of the HGFI, finally, the catalytic activity of the trypsin on the surface of the hydrophobic carrier is improved, the service life of the trypsin is prolonged, and the frequency of using of the trypsin is increased. The application of the trypsin to scientific researches and industries is improved.