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发明公开
CN106011299A 检测小麦中顶芒山羊草2M、3M、6M和7M染色体的特异分子标记、试剂盒及方法 有权

检测小麦中顶芒山羊草2M、3M、6M和7M染色体的特异分子标记、试剂盒及方法
摘要：
本发明涉及分子生物学领域，特别涉及检测小麦中顶芒山羊草2M、3M、6M和7M染色体的特异分子标记，分别如序列表中SEQ ID No.1和‑18所示的DNA碱基序列。将待检染色体样品与特异分子标记组合物或者放入试剂盒中进行扩增。在检测或辅助检测小麦是否含有顶芒山羊草2M、3M、6M和7M染色体。本发明对涉及顶芒山羊草2M、3M、6M和7M染色体的小麦‑顶芒山羊草远缘杂交新种质的筛选与鉴定提供了有效的检测手段；对以染色体片段的方式向小麦转移顶芒山羊草2M、3M、6M和7M染色体上的优异基因提供了检测方法，具有积极的产业化价值。
摘要（英）：

The invention relates to the field of molecular biology, in particular to specific molecular markers for detection of Aegilops comosa 2M, 3M, 6M and 7M chromosomes in wheat, and the specific molecular markers are DNA base sequences respectively shown as SEQ ID No.1 and -18 in the sequence table. A to-be-detected chromosome sample and a specific molecular marker composition are put into a kit to conduct amplification. The specific molecular markers and the kit can be used for detection or assisted detection of the existence of Aegilops comosa 2M, 3M, 6M and 7M chromosomes in wheat. The invention provides an effective detection means for screening and identification of wheat-Aegilops comosa distant hybridization new germplasms involving Aegilops comosa 2M, 3M, 6M and 7M chromosomes, provides a detection method for transfer of excellent genes on Aegilops comosa 2M, 3M, 6M and 7M chromosomes to wheat by means of chromosome segments, and has positive industrialization value.

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