The invention provides a knitting device of medical fabric, and belongs to the technical field of machines. The knitting device of the medical fabric solves the technical problems that in the prior art, because no fastening devices are arranged between material barrels in a warp knitting machine, the material barrels shake in the cloth winding process and the winding effect is poor, and the technical problems that in the prior art, because no pulling devices are arranged on the material barrels, the heavy material barrels need to be carried manually, labor intensity is high and production efficiency is low. The knitting device of the medical fabric comprises a machine frame and further comprises two supporting frames, a supporting shaft, a storage roller and a pre-tightening piece, wherein the two supporting frames are fixedly connected to the upper side of the machine frame, the two ends of the supporting shaft are connected to the corresponding supporting frames respectively, the storage roller is arranged on the supporting shaft in a sleeving mode and fixedly connected with the supporting shaft in the circumferential direction, and the pre-tightening piece is arranged on the supporting shaft in a sleeving mode and fixes the storage roller to the supporting shaft in the axial direction. A driving piece used for driving the supporting shaft to rotate is further arranged on the machine frame. The knitting device of the medical fabric has the advantages of being good in winding effect and high in production efficiency.