The invention discloses a preparation method of a novel cement base terrazzo floor. The novel cement base terrazzo floor adopts the following raw materials: cement, mineral powder, sand, stone and a swelling agent, wherein the mass ratio of the cement to the mineral powder to the sand to the stone to the swelling agent is (9-12):(1-3):(15-18):(25-29):1; the cement and mineral powder compose a binding material; and the water-binder ratio of the binding material is 0.29-0.34. The preparation method comprises the following steps: weighing the raw materials according to the proportion; mixing the raw materials to prepare cement base terrazzo; polishing the cement base terrazzo floor by using an adjustable high-speed polishing machine and a polishing agent; carrying out pore sealing treatment by using a permeable crystallization agent; and carrying out surface reinforcing treatment on the terrazzo with a nano surface reinforcing agent to obtain the novel cement base terrazzo floor. The preparation method can improve the strength of the terrazzo floor, improve the abrasion resistance of the terrazzo floor, improve the glossiness of the terrazzo floor, reduce the water absorption of the terrazzo floor and improve the anti-fouling property and anti-permeability of the surface layer.