The invention belongs to the electrochemical detection technical field, and discloses a molecularly imprinted electrochemical sensor for detecting bisphenol A and a preparation method and an application thereof. A glassy carbon electrode modified by a gold and reduced graphene oxide nano composite material is used as a working electrode, a poly-o-phenylenediamine and bisphenol A mixed film is prepared by cyclic voltammetry, and finally, the bisphenol A is eluted to obtain the molecularly imprinted electrochemical sensor. The content of bisphenol A in a soil sample is detected by differential pulse voltammetry, and compared with a high performance liquid chromatography method, the methods have good correlation. The imprinted electrochemical sensor has the characteristics of fast response, high sensitivity, strong selectivity, wider linear range, high practicability, easy popularization and the like.