The invention discloses a transparent conductive film manufacturing method, which comprises steps of substrate cleaning, PEDOT:PSS thin film coating, drying, modified fluid coating, secondary heating, cooling and inspection. The transparent substrate is made of soda-lime glass or PET flexible material. The modified fluid is composed of the following materials: 5 to 20 mg of 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid, 0.1 to 0.2 ml of dimethyl sulfoxide, and 0.8 to 0.9 ml of methanol. The secondary water adopted for cleaning is deionized water of 18 meghm. The method of the invention has the advantages of simple manufacturing, low material cost and easy process production. The conductivity of the manufactured transparent conductive film is greatly improved, an ITO conductive thin film can be replaced, and the transparent conductive film can be used as an electrode.