The invention discloses a simulating chamber for simulating blood circulation in vitro and an implementing method thereof. The simulating chamber comprises a linear motor, a motor spindle, a piston, a diaphragm, a gas chamber, a pressure communicating hole, a liquid level sensor, a pressure sensor, a volume control connecting opening, a test equipment connecting port, a two-way pump and a liquid chamber. The simulating chamber and a blood circulation system numerical model are connected through a data collection interface, pressure in the simulating chamber is fed back and controlled through software to follow reference pressure, generated by the numerical model, of the standard heart ventricle or aortas, and meanwhile a volume controller is arranged in the software to control liquid volume in a maintaining chamber of the two-way pump to be a set value. The simulating chamber can simulate multiple circulatory system chambers including the left and right heart ventricles, left and right atrium cordis, aortas and pulmonary arteries in the normal physiological and pathological states, can be used for in-vitro performance tests of angiocarpy equipment including left and right heart ventricle auxiliary devices and cardiac valves, and is diversified in function.